brocc plugin issue

Library Plugin Support
1

I keep getting the following error when running the brocc plugin：
qiime brocc classify-brocc --i-query query.qza --o-classification query-brocc.qza
/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/numpy/core/getlimits.py:542: UserWarning: Signature b'\x00\xd0\xcc\xcc\xcc\xcc\xcc\xcc\xfb\xbf\x00\x00\x00\x00\x00\x00' for <class 'numpy.longdouble'> does not match any known type: falling back to type probe function.
This warnings indicates broken support for the dtype!
machar = _get_machar(dtype)
Plugin error from brocc:

Command '['blastn', '-query', '/tmp/qiime2/vk1602/data/17760cc7-73da-4f13-bbde-a164e83a5bcf/data/dna-sequences.fasta', '-evalue', '1e-05', '-outfmt', '7', '-db', 'nt', '-max_target_seqs', '100', '-out', '/tmp/tmp05vd_fra']' returned non-zero exit status 3.

Debug info has been saved to /tmp/qiime2-q2cli-err-fnj3h2g_.log.

This is the problem log:
cat /tmp/qiime2-q2cli-err-fnj3h2g_.log
BLAST Database error: Database memory map file error
Traceback (most recent call last):
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/q2cli/commands.py", line 520, in call
results = self._execute_action(
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/q2cli/commands.py", line 581, in _execute_action
results = action(**arguments)
File "", line 2, in classify_brocc
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/qiime2/sdk/action.py", line 342, in bound_callable
outputs = self.callable_executor(
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/qiime2/sdk/action.py", line 576, in callable_executor
output_views = self._callable(**view_args)
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/site-packages/q2_brocc/plugin_setup.py", line 59, in classify_brocc
subprocess.run(blast_cmd, check=True)
File "/home/vk1602/anaconda3/envs/qiime2-amplicon-2024.5/lib/python3.9/subprocess.py", line 528, in run
raise CalledProcessError(retcode, process.args,
subprocess.CalledProcessError: Command '['blastn', '-query', '/tmp/qiime2/vk1602/data/17760cc7-73da-4f13-bbde-a164e83a5bcf/data/dna-sequences.fasta', '-evalue', '1e-05', '-outfmt', '7', '-db', 'nt', '-max_target_seqs', '100', '-out', '/tmp/tmp05vd_fra']' returned non-zero exit status 3.

My nt database is in the following location:
/mnt/e/blast+/ntdatabase/

The environment variables are as follows:
echo $BLASTDB
/mnt/e/blast+/ntdatabase/

ls $BLASTDB
nt_database.00.nhr nt_database.178.nsq nt_database.266.nin nt_database.354.nhr
nt_database.00.nin nt_database.179.nhr nt_database.266.nsq nt_database.354.nin
nt_database.00.nsq nt_database.179.nin nt_database.267.nhr nt_database.354.nsq
nt_database.01.nhr nt_database.179.nsq nt_database.267.nin nt_database.355.nhr
nt_database.01.nin nt_database.18.nhr nt_database.267.nsq nt_database.355.nin
nt_database.01.nsq nt_database.18.nin nt_database.268.nhr nt_database.355.nsq
nt_database.02.nhr nt_database.18.nsq nt_database.268.nin nt_database.356.nhr
nt_database.02.nin nt_database.180.nhr nt_database.268.nsq nt_database.356.nin
nt_database.02.nsq nt_database.180.nin nt_database.269.nhr nt_database.356.nsq
nt_database.03.nhr nt_database.180.nsq nt_database.269.nin nt_database.357.nhr
nt_database.03.nin nt_database.181.nhr nt_database.269.nsq nt_database.357.nin
nt_database.03.nsq nt_database.181.nin nt_database.27.nhr nt_database.357.nsq
nt_database.04.nhr nt_database.181.nsq nt_database.27.nin nt_database.358.nhr
nt_database.04.nin nt_database.182.nhr nt_database.27.nsq nt_database.358.nin
nt_database.04.nsq nt_database.182.nin nt_database.270.nhr nt_database.358.nsq
nt_database.05.nhr nt_database.182.nsq nt_database.270.nin nt_database.359.nhr
nt_database.05.nin nt_database.183.nhr nt_database.270.nsq nt_database.359.nin
nt_database.05.nsq nt_database.183.nin nt_database.271.nhr nt_database.359.nsq
nt_database.06.nhr nt_database.183.nsq nt_database.271.nin nt_database.36.nhr
nt_database.06.nin nt_database.184.nhr nt_database.271.nsq nt_database.36.nin
nt_database.06.nsq nt_database.184.nin nt_database.272.nhr nt_database.36.nsq
nt_database.07.nhr nt_database.184.nsq nt_database.272.nin nt_database.360.nhr
nt_database.07.nin nt_database.185.nhr nt_database.272.nsq nt_database.360.nin
nt_database.07.nsq nt_database.185.nin nt_database.273.nhr nt_database.360.nsq
nt_database.08.nhr nt_database.185.nsq nt_database.273.nin nt_database.361.nhr
nt_database.08.nin nt_database.186.nhr nt_database.273.nsq nt_database.361.nin
nt_database.08.nsq nt_database.186.nin nt_database.274.nhr nt_database.361.nsq
nt_database.09.nhr nt_database.186.nsq nt_database.274.nin nt_database.362.nhr
nt_database.09.nin nt_database.187.nhr nt_database.274.nsq nt_database.362.nin
nt_database.09.nsq nt_database.187.nin nt_database.275.nhr nt_database.362.nsq
nt_database.10.nhr nt_database.187.nsq nt_database.275.nin nt_database.363.nhr
nt_database.10.nin nt_database.188.nhr nt_database.275.nsq nt_database.363.nin
nt_database.10.nsq nt_database.188.nin nt_database.276.nhr nt_database.363.nsq
nt_database.100.nhr nt_database.188.nsq nt_database.276.nin nt_database.364.nhr
nt_database.100.nin nt_database.189.nhr nt_database.276.nsq nt_database.364.nin
nt_database.100.nsq nt_database.189.nin nt_database.277.nhr nt_database.364.nsq
nt_database.101.nhr nt_database.189.nsq nt_database.277.nin nt_database.365.nhr
nt_database.101.nin nt_database.19.nhr nt_database.277.nsq nt_database.365.nin
nt_database.101.nsq nt_database.19.nin nt_database.278.nhr nt_database.365.nsq
nt_database.102.nhr nt_database.19.nsq nt_database.278.nin nt_database.366.nhr
nt_database.102.nin nt_database.190.nhr nt_database.278.nsq nt_database.366.nin
nt_database.102.nsq nt_database.190.nin nt_database.279.nhr nt_database.366.nsq
nt_database.103.nhr nt_database.190.nsq nt_database.279.nin nt_database.367.nhr
nt_database.103.nin nt_database.191.nhr nt_database.279.nsq nt_database.367.nin
nt_database.103.nsq nt_database.191.nin nt_database.28.nhr nt_database.367.nsq
nt_database.104.nhr nt_database.191.nsq nt_database.28.nin nt_database.368.nhr
nt_database.104.nin nt_database.192.nhr nt_database.28.nsq nt_database.368.nin
nt_database.104.nsq nt_database.192.nin nt_database.280.nhr nt_database.368.nsq
nt_database.105.nhr nt_database.192.nsq nt_database.280.nin nt_database.369.nhr
nt_database.105.nin nt_database.193.nhr nt_database.280.nsq nt_database.369.nin
nt_database.105.nsq nt_database.193.nin nt_database.281.nhr nt_database.369.nsq
nt_database.106.nhr nt_database.193.nsq nt_database.281.nin nt_database.37.nhr
nt_database.106.nin nt_database.194.nhr nt_database.281.nsq nt_database.37.nin
nt_database.106.nsq nt_database.194.nin nt_database.282.nhr nt_database.37.nsq
nt_database.107.nhr nt_database.194.nsq nt_database.282.nin nt_database.370.nhr
nt_database.107.nin nt_database.195.nhr nt_database.282.nsq nt_database.370.nin
nt_database.107.nsq nt_database.195.nin nt_database.283.nhr nt_database.370.nsq
nt_database.108.nhr nt_database.195.nsq nt_database.283.nin nt_database.371.nhr
nt_database.108.nin nt_database.196.nhr nt_database.283.nsq nt_database.371.nin
nt_database.108.nsq nt_database.196.nin nt_database.284.nhr nt_database.371.nsq
nt_database.109.nhr nt_database.196.nsq nt_database.284.nin nt_database.372.nhr
nt_database.109.nin nt_database.197.nhr nt_database.284.nsq nt_database.372.nin
nt_database.109.nsq nt_database.197.nin nt_database.285.nhr nt_database.372.nsq
nt_database.11.nhr nt_database.197.nsq nt_database.285.nin nt_database.373.nhr
nt_database.11.nin nt_database.198.nhr nt_database.285.nsq nt_database.373.nin
nt_database.11.nsq nt_database.198.nin nt_database.286.nhr nt_database.373.nsq
nt_database.110.nhr nt_database.198.nsq nt_database.286.nin nt_database.374.nhr
nt_database.110.nin nt_database.199.nhr nt_database.286.nsq nt_database.374.nin
nt_database.110.nsq nt_database.199.nin nt_database.287.nhr nt_database.374.nsq
nt_database.111.nhr nt_database.199.nsq nt_database.287.nin nt_database.375.nhr
nt_database.111.nin nt_database.20.nhr nt_database.287.nsq nt_database.375.nin
nt_database.111.nsq nt_database.20.nin nt_database.288.nhr nt_database.375.nsq
nt_database.112.nhr nt_database.20.nsq nt_database.288.nin nt_database.376.nhr
nt_database.112.nin nt_database.200.nhr nt_database.288.nsq nt_database.376.nin
nt_database.112.nsq nt_database.200.nin nt_database.289.nhr nt_database.376.nsq
nt_database.113.nhr nt_database.200.nsq nt_database.289.nin nt_database.377.nhr
nt_database.113.nin nt_database.201.nhr nt_database.289.nsq nt_database.377.nin
nt_database.113.nsq nt_database.201.nin nt_database.29.nhr nt_database.377.nsq
nt_database.114.nhr nt_database.201.nsq nt_database.29.nin nt_database.378.nhr
nt_database.114.nin nt_database.202.nhr nt_database.29.nsq nt_database.378.nin
nt_database.114.nsq nt_database.202.nin nt_database.290.nhr nt_database.378.nsq
nt_database.115.nhr nt_database.202.nsq nt_database.290.nin nt_database.379.nhr
nt_database.115.nin nt_database.203.nhr nt_database.290.nsq nt_database.379.nin
nt_database.115.nsq nt_database.203.nin nt_database.291.nhr nt_database.379.nsq
nt_database.116.nhr nt_database.203.nsq nt_database.291.nin nt_database.38.nhr
nt_database.116.nin nt_database.204.nhr nt_database.291.nsq nt_database.38.nin
nt_database.116.nsq nt_database.204.nin nt_database.292.nhr nt_database.38.nsq
nt_database.117.nhr nt_database.204.nsq nt_database.292.nin nt_database.380.nhr
nt_database.117.nin nt_database.205.nhr nt_database.292.nsq nt_database.380.nin
nt_database.117.nsq nt_database.205.nin nt_database.293.nhr nt_database.380.nsq
nt_database.118.nhr nt_database.205.nsq nt_database.293.nin nt_database.381.nhr
nt_database.118.nin nt_database.206.nhr nt_database.293.nsq nt_database.381.nin
nt_database.118.nsq nt_database.206.nin nt_database.294.nhr nt_database.381.nsq
nt_database.119.nhr nt_database.206.nsq nt_database.294.nin nt_database.382.nhr
nt_database.119.nin nt_database.207.nhr nt_database.294.nsq nt_database.382.nin
nt_database.119.nsq nt_database.207.nin nt_database.295.nhr nt_database.382.nsq
nt_database.12.nhr nt_database.207.nsq nt_database.295.nin nt_database.383.nhr
nt_database.12.nin nt_database.208.nhr nt_database.295.nsq nt_database.383.nin
nt_database.12.nsq nt_database.208.nin nt_database.296.nhr nt_database.383.nsq
nt_database.120.nhr nt_database.208.nsq nt_database.296.nin nt_database.384.nhr
nt_database.120.nin nt_database.209.nhr nt_database.296.nsq nt_database.384.nin
nt_database.120.nsq nt_database.209.nin nt_database.297.nhr nt_database.384.nsq
nt_database.121.nhr nt_database.209.nsq nt_database.297.nin nt_database.39.nhr
nt_database.121.nin nt_database.21.nhr nt_database.297.nsq nt_database.39.nin
nt_database.121.nsq nt_database.21.nin nt_database.298.nhr nt_database.39.nsq
nt_database.122.nhr nt_database.21.nsq nt_database.298.nin nt_database.40.nhr
nt_database.122.nin nt_database.210.nhr nt_database.298.nsq nt_database.40.nin
nt_database.122.nsq nt_database.210.nin nt_database.299.nhr nt_database.40.nsq
nt_database.123.nhr nt_database.210.nsq nt_database.299.nin nt_database.41.nhr
nt_database.123.nin nt_database.211.nhr nt_database.299.nsq nt_database.41.nin
nt_database.123.nsq nt_database.211.nin nt_database.30.nhr nt_database.41.nsq
nt_database.124.nhr nt_database.211.nsq nt_database.30.nin nt_database.42.nhr
nt_database.124.nin nt_database.212.nhr nt_database.30.nsq nt_database.42.nin
nt_database.124.nsq nt_database.212.nin nt_database.300.nhr nt_database.42.nsq
nt_database.125.nhr nt_database.212.nsq nt_database.300.nin nt_database.43.nhr
nt_database.125.nin nt_database.213.nhr nt_database.300.nsq nt_database.43.nin
nt_database.125.nsq nt_database.213.nin nt_database.301.nhr nt_database.43.nsq
nt_database.126.nhr nt_database.213.nsq nt_database.301.nin nt_database.44.nhr
nt_database.126.nin nt_database.214.nhr nt_database.301.nsq nt_database.44.nin
nt_database.126.nsq nt_database.214.nin nt_database.302.nhr nt_database.44.nsq
nt_database.127.nhr nt_database.214.nsq nt_database.302.nin nt_database.45.nhr
nt_database.127.nin nt_database.215.nhr nt_database.302.nsq nt_database.45.nin
nt_database.127.nsq nt_database.215.nin nt_database.303.nhr nt_database.45.nsq
nt_database.128.nhr nt_database.215.nsq nt_database.303.nin nt_database.46.nhr
nt_database.128.nin nt_database.216.nhr nt_database.303.nsq nt_database.46.nin
nt_database.128.nsq nt_database.216.nin nt_database.304.nhr nt_database.46.nsq
nt_database.129.nhr nt_database.216.nsq nt_database.304.nin nt_database.47.nhr
nt_database.129.nin nt_database.217.nhr nt_database.304.nsq nt_database.47.nin
nt_database.129.nsq nt_database.217.nin nt_database.305.nhr nt_database.47.nsq
nt_database.13.nhr nt_database.217.nsq nt_database.305.nin nt_database.48.nhr
nt_database.13.nin nt_database.218.nhr nt_database.305.nsq nt_database.48.nin
nt_database.13.nsq nt_database.218.nin nt_database.306.nhr nt_database.48.nsq
nt_database.130.nhr nt_database.218.nsq nt_database.306.nin nt_database.49.nhr
nt_database.130.nin nt_database.219.nhr nt_database.306.nsq nt_database.49.nin
nt_database.130.nsq nt_database.219.nin nt_database.307.nhr nt_database.49.nsq
nt_database.131.nhr nt_database.219.nsq nt_database.307.nin nt_database.50.nhr
nt_database.131.nin nt_database.22.nhr nt_database.307.nsq nt_database.50.nin
nt_database.131.nsq nt_database.22.nin nt_database.308.nhr nt_database.50.nsq
nt_database.132.nhr nt_database.22.nsq nt_database.308.nin nt_database.51.nhr
nt_database.132.nin nt_database.220.nhr nt_database.308.nsq nt_database.51.nin
nt_database.132.nsq nt_database.220.nin nt_database.309.nhr nt_database.51.nsq
nt_database.133.nhr nt_database.220.nsq nt_database.309.nin nt_database.52.nhr
nt_database.133.nin nt_database.221.nhr nt_database.309.nsq nt_database.52.nin
nt_database.133.nsq nt_database.221.nin nt_database.31.nhr nt_database.52.nsq
nt_database.134.nhr nt_database.221.nsq nt_database.31.nin nt_database.53.nhr
nt_database.134.nin nt_database.222.nhr nt_database.31.nsq nt_database.53.nin
nt_database.134.nsq nt_database.222.nin nt_database.310.nhr nt_database.53.nsq
nt_database.135.nhr nt_database.222.nsq nt_database.310.nin nt_database.54.nhr
nt_database.135.nin nt_database.223.nhr nt_database.310.nsq nt_database.54.nin
nt_database.135.nsq nt_database.223.nin nt_database.311.nhr nt_database.54.nsq
nt_database.136.nhr nt_database.223.nsq nt_database.311.nin nt_database.55.nhr
nt_database.136.nin nt_database.224.nhr nt_database.311.nsq nt_database.55.nin
nt_database.136.nsq nt_database.224.nin nt_database.312.nhr nt_database.55.nsq
nt_database.137.nhr nt_database.224.nsq nt_database.312.nin nt_database.56.nhr
nt_database.137.nin nt_database.225.nhr nt_database.312.nsq nt_database.56.nin
nt_database.137.nsq nt_database.225.nin nt_database.313.nhr nt_database.56.nsq
nt_database.138.nhr nt_database.225.nsq nt_database.313.nin nt_database.57.nhr
nt_database.138.nin nt_database.226.nhr nt_database.313.nsq nt_database.57.nin
nt_database.138.nsq nt_database.226.nin nt_database.314.nhr nt_database.57.nsq
nt_database.139.nhr nt_database.226.nsq nt_database.314.nin nt_database.58.nhr
nt_database.139.nin nt_database.227.nhr nt_database.314.nsq nt_database.58.nin
nt_database.139.nsq nt_database.227.nin nt_database.315.nhr nt_database.58.nsq
nt_database.14.nhr nt_database.227.nsq nt_database.315.nin nt_database.59.nhr
nt_database.14.nin nt_database.228.nhr nt_database.315.nsq nt_database.59.nin
nt_database.14.nsq nt_database.228.nin nt_database.316.nhr nt_database.59.nsq
nt_database.140.nhr nt_database.228.nsq nt_database.316.nin nt_database.60.nhr
nt_database.140.nin nt_database.229.nhr nt_database.316.nsq nt_database.60.nin
nt_database.140.nsq nt_database.229.nin nt_database.317.nhr nt_database.60.nsq
nt_database.141.nhr nt_database.229.nsq nt_database.317.nin nt_database.61.nhr
nt_database.141.nin nt_database.23.nhr nt_database.317.nsq nt_database.61.nin
nt_database.141.nsq nt_database.23.nin nt_database.318.nhr nt_database.61.nsq
nt_database.142.nhr nt_database.23.nsq nt_database.318.nin nt_database.62.nhr
nt_database.142.nin nt_database.230.nhr nt_database.318.nsq nt_database.62.nin
nt_database.142.nsq nt_database.230.nin nt_database.319.nhr nt_database.62.nsq
nt_database.143.nhr nt_database.230.nsq nt_database.319.nin nt_database.63.nhr
nt_database.143.nin nt_database.231.nhr nt_database.319.nsq nt_database.63.nin
nt_database.143.nsq nt_database.231.nin nt_database.32.nhr nt_database.63.nsq
nt_database.144.nhr nt_database.231.nsq nt_database.32.nin nt_database.64.nhr
nt_database.144.nin nt_database.232.nhr nt_database.32.nsq nt_database.64.nin
nt_database.144.nsq nt_database.232.nin nt_database.320.nhr nt_database.64.nsq
nt_database.145.nhr nt_database.232.nsq nt_database.320.nin nt_database.65.nhr
nt_database.145.nin nt_database.233.nhr nt_database.320.nsq nt_database.65.nin
nt_database.145.nsq nt_database.233.nin nt_database.321.nhr nt_database.65.nsq
nt_database.146.nhr nt_database.233.nsq nt_database.321.nin nt_database.66.nhr
nt_database.146.nin nt_database.234.nhr nt_database.321.nsq nt_database.66.nin
nt_database.146.nsq nt_database.234.nin nt_database.322.nhr nt_database.66.nsq
nt_database.147.nhr nt_database.234.nsq nt_database.322.nin nt_database.67.nhr
nt_database.147.nin nt_database.235.nhr nt_database.322.nsq nt_database.67.nin
nt_database.147.nsq nt_database.235.nin nt_database.323.nhr nt_database.67.nsq
nt_database.148.nhr nt_database.235.nsq nt_database.323.nin nt_database.68.nhr
nt_database.148.nin nt_database.236.nhr nt_database.323.nsq nt_database.68.nin
nt_database.148.nsq nt_database.236.nin nt_database.324.nhr nt_database.68.nsq
nt_database.149.nhr nt_database.236.nsq nt_database.324.nin nt_database.69.nhr
nt_database.149.nin nt_database.237.nhr nt_database.324.nsq nt_database.69.nin
nt_database.149.nsq nt_database.237.nin nt_database.325.nhr nt_database.69.nsq
nt_database.15.nhr nt_database.237.nsq nt_database.325.nin nt_database.70.nhr
nt_database.15.nin nt_database.238.nhr nt_database.325.nsq nt_database.70.nin
nt_database.15.nsq nt_database.238.nin nt_database.326.nhr nt_database.70.nsq
nt_database.150.nhr nt_database.238.nsq nt_database.326.nin nt_database.71.nhr
nt_database.150.nin nt_database.239.nhr nt_database.326.nsq nt_database.71.nin
nt_database.150.nsq nt_database.239.nin nt_database.327.nhr nt_database.71.nsq
nt_database.151.nhr nt_database.239.nsq nt_database.327.nin nt_database.72.nhr
nt_database.151.nin nt_database.24.nhr nt_database.327.nsq nt_database.72.nin
nt_database.151.nsq nt_database.24.nin nt_database.328.nhr nt_database.72.nsq
nt_database.152.nhr nt_database.24.nsq nt_database.328.nin nt_database.73.nhr
nt_database.152.nin nt_database.240.nhr nt_database.328.nsq nt_database.73.nin
nt_database.152.nsq nt_database.240.nin nt_database.329.nhr nt_database.73.nsq
nt_database.153.nhr nt_database.240.nsq nt_database.329.nin nt_database.74.nhr
nt_database.153.nin nt_database.241.nhr nt_database.329.nsq nt_database.74.nin
nt_database.153.nsq nt_database.241.nin nt_database.33.nhr nt_database.74.nsq
nt_database.154.nhr nt_database.241.nsq nt_database.33.nin nt_database.75.nhr
nt_database.154.nin nt_database.242.nhr nt_database.33.nsq nt_database.75.nin
nt_database.154.nsq nt_database.242.nin nt_database.330.nhr nt_database.75.nsq
nt_database.155.nhr nt_database.242.nsq nt_database.330.nin nt_database.76.nhr
nt_database.155.nin nt_database.243.nhr nt_database.330.nsq nt_database.76.nin
nt_database.155.nsq nt_database.243.nin nt_database.331.nhr nt_database.76.nsq
nt_database.156.nhr nt_database.243.nsq nt_database.331.nin nt_database.77.nhr
nt_database.156.nin nt_database.244.nhr nt_database.331.nsq nt_database.77.nin
nt_database.156.nsq nt_database.244.nin nt_database.332.nhr nt_database.77.nsq
nt_database.157.nhr nt_database.244.nsq nt_database.332.nin nt_database.78.nhr
nt_database.157.nin nt_database.245.nhr nt_database.332.nsq nt_database.78.nin
nt_database.157.nsq nt_database.245.nin nt_database.333.nhr nt_database.78.nsq
nt_database.158.nhr nt_database.245.nsq nt_database.333.nin nt_database.79.nhr
nt_database.158.nin nt_database.246.nhr nt_database.333.nsq nt_database.79.nin
nt_database.158.nsq nt_database.246.nin nt_database.334.nhr nt_database.79.nsq
nt_database.159.nhr nt_database.246.nsq nt_database.334.nin nt_database.80.nhr
nt_database.159.nin nt_database.247.nhr nt_database.334.nsq nt_database.80.nin
nt_database.159.nsq nt_database.247.nin nt_database.335.nhr nt_database.80.nsq
nt_database.16.nhr nt_database.247.nsq nt_database.335.nin nt_database.81.nhr
nt_database.16.nin nt_database.248.nhr nt_database.335.nsq nt_database.81.nin
nt_database.16.nsq nt_database.248.nin nt_database.336.nhr nt_database.81.nsq
nt_database.160.nhr nt_database.248.nsq nt_database.336.nin nt_database.82.nhr
nt_database.160.nin nt_database.249.nhr nt_database.336.nsq nt_database.82.nin
nt_database.160.nsq nt_database.249.nin nt_database.337.nhr nt_database.82.nsq
nt_database.161.nhr nt_database.249.nsq nt_database.337.nin nt_database.83.nhr
nt_database.161.nin nt_database.25.nhr nt_database.337.nsq nt_database.83.nin
nt_database.161.nsq nt_database.25.nin nt_database.338.nhr nt_database.83.nsq
nt_database.162.nhr nt_database.25.nsq nt_database.338.nin nt_database.84.nhr
nt_database.162.nin nt_database.250.nhr nt_database.338.nsq nt_database.84.nin
nt_database.162.nsq nt_database.250.nin nt_database.339.nhr nt_database.84.nsq
nt_database.163.nhr nt_database.250.nsq nt_database.339.nin nt_database.85.nhr
nt_database.163.nin nt_database.251.nhr nt_database.339.nsq nt_database.85.nin
nt_database.163.nsq nt_database.251.nin nt_database.34.nhr nt_database.85.nsq
nt_database.164.nhr nt_database.251.nsq nt_database.34.nin nt_database.86.nhr
nt_database.164.nin nt_database.252.nhr nt_database.34.nsq nt_database.86.nin
nt_database.164.nsq nt_database.252.nin nt_database.340.nhr nt_database.86.nsq
nt_database.165.nhr nt_database.252.nsq nt_database.340.nin nt_database.87.nhr
nt_database.165.nin nt_database.253.nhr nt_database.340.nsq nt_database.87.nin
nt_database.165.nsq nt_database.253.nin nt_database.341.nhr nt_database.87.nsq
nt_database.166.nhr nt_database.253.nsq nt_database.341.nin nt_database.88.nhr
nt_database.166.nin nt_database.254.nhr nt_database.341.nsq nt_database.88.nin
nt_database.166.nsq nt_database.254.nin nt_database.342.nhr nt_database.88.nsq
nt_database.167.nhr nt_database.254.nsq nt_database.342.nin nt_database.89.nhr
nt_database.167.nin nt_database.255.nhr nt_database.342.nsq nt_database.89.nin
nt_database.167.nsq nt_database.255.nin nt_database.343.nhr nt_database.89.nsq
nt_database.168.nhr nt_database.255.nsq nt_database.343.nin nt_database.90.nhr
nt_database.168.nin nt_database.256.nhr nt_database.343.nsq nt_database.90.nin
nt_database.168.nsq nt_database.256.nin nt_database.344.nhr nt_database.90.nsq
nt_database.169.nhr nt_database.256.nsq nt_database.344.nin nt_database.91.nhr
nt_database.169.nin nt_database.257.nhr nt_database.344.nsq nt_database.91.nin
nt_database.169.nsq nt_database.257.nin nt_database.345.nhr nt_database.91.nsq
nt_database.17.nhr nt_database.257.nsq nt_database.345.nin nt_database.92.nhr
nt_database.17.nin nt_database.258.nhr nt_database.345.nsq nt_database.92.nin
nt_database.17.nsq nt_database.258.nin nt_database.346.nhr nt_database.92.nsq
nt_database.170.nhr nt_database.258.nsq nt_database.346.nin nt_database.93.nhr
nt_database.170.nin nt_database.259.nhr nt_database.346.nsq nt_database.93.nin
nt_database.170.nsq nt_database.259.nin nt_database.347.nhr nt_database.93.nsq
nt_database.171.nhr nt_database.259.nsq nt_database.347.nin nt_database.94.nhr
nt_database.171.nin nt_database.26.nhr nt_database.347.nsq nt_database.94.nin
nt_database.171.nsq nt_database.26.nin nt_database.348.nhr nt_database.94.nsq
nt_database.172.nhr nt_database.26.nsq nt_database.348.nin nt_database.95.nhr
nt_database.172.nin nt_database.260.nhr nt_database.348.nsq nt_database.95.nin
nt_database.172.nsq nt_database.260.nin nt_database.349.nhr nt_database.95.nsq
nt_database.173.nhr nt_database.260.nsq nt_database.349.nin nt_database.96.nhr
nt_database.173.nin nt_database.261.nhr nt_database.349.nsq nt_database.96.nin
nt_database.173.nsq nt_database.261.nin nt_database.35.nhr nt_database.96.nsq
nt_database.174.nhr nt_database.261.nsq nt_database.35.nin nt_database.97.nhr
nt_database.174.nin nt_database.262.nhr nt_database.35.nsq nt_database.97.nin
nt_database.174.nsq nt_database.262.nin nt_database.350.nhr nt_database.97.nsq
nt_database.175.nhr nt_database.262.nsq nt_database.350.nin nt_database.98.nhr
nt_database.175.nin nt_database.263.nhr nt_database.350.nsq nt_database.98.nin
nt_database.175.nsq nt_database.263.nin nt_database.351.nhr nt_database.98.nsq
nt_database.176.nhr nt_database.263.nsq nt_database.351.nin nt_database.99.nhr
nt_database.176.nin nt_database.264.nhr nt_database.351.nsq nt_database.99.nin
nt_database.176.nsq nt_database.264.nin nt_database.352.nhr nt_database.99.nsq
nt_database.177.nhr nt_database.264.nsq nt_database.352.nin nt_database.nal
nt_database.177.nin nt_database.265.nhr nt_database.352.nsq results.out
nt_database.177.nsq nt_database.265.nin nt_database.353.nhr
nt_database.178.nhr nt_database.265.nsq nt_database.353.nin
nt_database.178.nin nt_database.266.nhr nt_database.353.nsq

The code keeps bugging. I need help,thank you

4

Hi @vk1602,
I am not familiar with Brooc

I think this is a out of memory error that you are getting here. From what I understand the NT database is very large.
Have you tried running this on a smaller database? Like Standard+plants+Fungi? Something like that would be closer to 80G.

1 Like
5

NCBI started making amplicon databases!

https://www.ncbi.nlm.nih.gov/refseq/targetedloci/

Depending on what region you sequenced, check out the Fungi RefSeq ITS project
https://www.ncbi.nlm.nih.gov/refseq/targetedloci/ITS_process/

1 Like
6

thank you，I didn't try to run it on a small database,because this query.qza file is the one I downloaded for testing, and I want to use this file to test whether the brocc plugin was installed successfully,I need nt database to analyze my 18s data and I have analyzed this file using the blastn command alone, and it can be analyzed

7

I reread your post and noticed this:

Here's how we are calling blastn internally.

According to the NCBI blast docs,

3 Error in BLAST engine

This is NOT one of the other errors, so hopefully the query sequences, database sequences, and memory are all fine.

Perhaps investigating this error may be helpful.

8

I checked my database，and there's nothing wrong

blastdbcheck -db /mnt/e/blast+/ntdatabase/nt_database
Writing messages to at verbosity (Summary)
ISAM testing is ENABLED.
Legacy testing is DISABLED.
TaxID testing is DISABLED.
By default, testing 200 randomly sampled OIDs.

Testing 385 volume(s).
Result=SUCCESS. No errors reported for 385 volume(s).
Testing 1 alias(es).
Result=SUCCESS. No errors reported for 1 alias(es).

And I ran the blastn program alone, and it works

9

Try the suggestion from this thread: qiime feature-classifier classify-consensus-blast

I also Searched for '3 Error in BLAST engine' and found this:

That programer makes a few suggestions that you could try.

You have discovered an uncommon error! :trophy:

Thank you for bringing this to our attention and helping us understand why it's happening.

10

TaxID testing is DISABLED.
So... maybe it's an issue there? That matches the recommendation from GitHub...

Let us know what you find!

1 Like
12

thank you

This is the command line where I set up the database

makeblastdb
--in nt
--dbtype nucl
--out nt_database

I asked GTP, it told me there are no options specified in the command to include TaxID information (such as -parse_seqids or -taxid_map). This is probably what caused me to fail to get a TaxID when using blastdbcmd.I'm going to try to reconstruct the database and see if I can solve this problem

13

I see! Good thinking to use an LLM for troubleshooting!

Next time you try running qiime, include --verbose and post the result. If blast itself is working, which it looks like it is, there may be more clues from inside our pipeline.

14

I solved this problem by adding a path command to the database so that qiime2 can find the directory of the database，like this：

 time qiime brocc classify-brocc \
  --i-query query.qza \
  --o-classification query-ceshi-brocc.qza \
  --p-blastdb /mnt/e/blast+/ntdatabase/nt_database
2 Likes