Hello guys
I got a taxonomy data and now i want to proceed LEfSe analysis.
I want to know what data usually use to input file.
Are you using the raw sequence count obtained as a result of taxonomy? Or do you use data converted to ratio (%, relative abundance)?
Overall ratio of the control group and the treatment group should be compared, so the ratio is correct, right?
I am a newbie in microbiome analysis, please help me!