Barcodes problem in demultiplexing with q2-cutadpt

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1

Hello!

When I use q2-cutadpt demux-paired with 6 bases long barcodes, sequences are gathered in just one or two samples, and other samples hold fewer sequences.
demux-odd.qzv (298.2 KB)

I guess it's because the barcodes are too short and they appear in some sequences. So I add the first 6 bases of forward primer to every barcode, and they are combined to a 12 bases “barcode”. Then the demultiplex result seems correct.
demux.qzv (295.4 KB)

I wonder whether my guess and treat is correct. If yes, maybe I should re-process my previous short barcodes sequences.:cry:

Thank you!

3

Hi @zArctander!

Have you tried “anchoring” the barcode?

#SampleID    Barcode
1       ^GAAGGC
2       ^ATGTCA
2D      ^CGGTTA
...
5

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