archaea , bacteria and the SILA database

Hi all. Just a quick question I hope someone can hekp with. Can i use SILVA 138 database for the V3 and V4 region of bacterial 16 S rRNA gene with primers Bakt_341F and Bakt_805R and the V6-V8 region of the archeal 16S rRNA with the primers A956F and A1401R.

Or does it require something different?

Best wishes

Hi @Mantella86,

Yep! You can either use the full-length SILVA SSU sequence database available on the Data resources page, or you can curate your own SILVA-based classifier with RESCRIPt for your target region of interest: