Good morning, I have some problem to running the DADA2 plugin. Sample is pairedend FASTQ file from ubiome company. Data information, sample_A_R1.fastq, sample_A_R2.fastq V4 region, 515F - 806R I run plugins in qiime2-2018.11. [Importing] qiime tools import --type 'SampleData[PairedEndSeq…

Hi @kyeong_yun ! Thanks for writing up a detailed post - unfortunately, your process is not entirely clear to me. A few syntax issues jump out (in 1, multiple trim-lefts, in 1, a --p-*tunc*-left), but I suspect this is not cut/pasted from your actual code. [image] kyeong_yun: –p-trim-left-f 10…

Thank you for the prompt reply . I turned dada2 plugin four times with the above options. When i turned it like this, the file was not created. qiime dada2 denoise-paired --demultiplexed-seqs CutAdapt/paired-end-demux-cutadapt.qza --p-trimed-left-f 10 --p-trimed-left-r 10 --p-trunc-len-f …

@kyeong_yun , sorry if my post was a bit long - if you can share the requested file it will be easier for me to help troubleshoot. [image] ChrisKeefe: How did you select your trim and trunc parameters? Can you share the DADA2 stats from the first run (in which the error message suggested you mod…

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