…
miniconda2/envs/qiime2/lib/python3.5/site-packages/q2_dada2/_denoise.py", line 45, in _check_featureless_table
raise ValueError("No features remain after denoising. Try adjusting "
ValueError: No features remain after denoising. Try adjusting your truncation and trim parameter settings.
Plugin error from dada2: No features remain after denoising. Try adjusting your truncation and trim parameter settings.
…
miniconda2/envs/qiime2/lib/python3.5/site-packages/q2_dada2/_denoise.py", line 246, in denoise_paired
" and stderr to learn more." % e.returncode)
Exception: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more. Plugin error from dada2: An error was encountered while running DADA2 in R (return code 1), please inspect stdout and stderr to learn more.
Hi @kyeong_yun!
Thanks for writing up a detailed post - unfortunately, your process is not entirely clear to me. A few syntax issues jump out (in 1, multiple trim-lefts, in 1, a --p-*tunc*-left), but I suspect this is not cut/pasted from your actual code.
How did you select your trim and trunc parameters? Can you share the DADA2 stats from the first run (in which the error message suggested you modify the parameters?
The demultiplexed sequence length has a value between 147 and 150 in paired-end-demux-cutadapt.qzv, the above option are selected.
And quality declined before 7 base.