I have been following the "Moving Pictures" tutorial to run my 16S analyzes.
However, when I ran the following command to analyze alpha diversity...
qiime diversity alpha-group-significance --i-alpha-diversity core-metrics-results/faith_pd_vector.qza --m-metadata-file sample-metadata.tsv --o-visualization core-metrics-results/faith-pd-group-significance.qzv
... I have gotten the following error:
The following categorical metadata columns have been omitted because the number of groups was equal to the number of samples, there was only a single group, or the column consisted only of missing data: sample
This is my sample metadata file: sample-metadata - Google Sheets. I guess the problem is that the number of groups is equal to the number of samples... am I right?
Anyway, I really would like to compare alpha diversity between the different samples! Is there a way to rearrange my sample sheet so I can do that? Or maybe run some analysis on QIIME2 through R...
Thank you in advance.