Hi everyone, Im I first-timer with qiime2, and Im having some problem with Alpha and beta diversity analysis. Im currently following the “Moving pictures” tutorial and for the following code
qiime diversity core-metrics-phylogenetic \
I obtain this error message
Plugin error from diversity:
Ordinations with less than two dimensions are not supported.
Debug info has been saved to /tmp/qiime2-q2cli-err-xcxjhkq9.log
I don’t understand where the mistake might be. Thank you all in advance.
Welcome to qiime2 forum!
Thats weird because the tutorial file is proved by many users. I believe one of your inputing file is broken. I suggest you re-download the table-file in the tutorial page and re-command to see if there still has error issue.
Im afraid I forgot to mention that Im working with my own data set.
Welcome to the forum @jose_gacia!
This error seems to suggest that you only have a single sample in your feature table. Is that correct?
If yes: don’t use this method, since beta diversity is only meaningful if you have ≥ 2 samples. You could still run
qiime diversity rarefy and
qiime diversity alpha to estimate alpha diversity on that single sample if that is what you are after.
if no: run
qiime feature-table summarize and please post the QZV here.
I hope that helps!
This is indeed the case, should have seen It before, so much to learn. Tank you very much!!!
This topic was automatically closed 31 days after the last reply. New replies are no longer allowed.