I am running the following code to filter features based on total frequency:
qiime feature-table filter-features --i-table 16s-merged-table.qza --p-min-frequency 3 --o-filtered-table 16s-table-gt3.qza qiime feature-table summarize --i-table 16s-table-gt3.qza --o-visualization 16s-table-gt3.qzv qiime feature-table filter-seqs --i-data 16s-merged-seqs.qza --i-table 16s-table-gt3.qza --o-filtered-data 16s-merged-seqs-gt3.qza qiime feature-table tabulate-seqs --i-data 16s-merged-seqs-gt3.qza --o-visualization 16s-merged-seqs-gt3.qzv
The first part, where I filter features from my feature table seems to work fine, but when I try to filter my sequences using the table, I get this error:
All features were filtered out of the data.
I have checked the filtered table, and it has data.
Any ideas on what’s going on? I read this post first, but since the user is filtering based on metadata and I am not, I don’t think the solution applies.
Here is my filtered table:16s-table-gt3.qzv (2.1 MB)
Here are the sequences that I am trying to filter: 16s-merged-seqs.qzv (800.0 KB)
Thanks for your help!