I would like to know after using DADA2 plugin, following by taking OTUs is necessary or clustering must be done? No need?
According to the cases I read I think it is not required, but I am in doubt:
- Reads should then be denoised into amplicon sequence variants (ASVs) or clustered into operational taxonomic units (OTUs).
- Algorithm (DADA), a model-based approach for correcting
amplicon errors without constructing OTUs5. DADA identified
fine-scale variation in 454-sequenced amplicon data while outputting few false positives