Hi everyone, not sure if this is the right sub-forum to post this question but does anyone have any experience in extracting DNA from mice fed high fat diets? I have been using a chloroform/phenol/alcohol extraction protocol to get some DNA but my yields are always unreasonably low leading to poor sequencing data. I suspect the high lipid content of the stool some how interferes with DNA extraction. I’ve seen the Powersoil kit mentioned quite a few times in the literature, does anyone have any experience using this and what kind of yields were you getting? Are there other good protocols I could use to circumvent this problem?