I analyzed data from human fecal samples generated with the 16s metagenomics kit using Ion Torrent sequencing. The equivalent of OTU table has been generated using the Software Ion Reporter (Thermo Scientific), which I did not done. The analyze diversity revealed lower diversity compared to my preceding analyze that realized with data generated with MiSeq 16s sequencing. I find about 120 bacterial species per human fecal samples instead of 800 Otus…
Any explanations ?
Somebody have a similar experiments ?
Thank you for your contribution.