16S analysis on my personal computer

Hello everyone!
I have a question regarding data analysis. Can I analyze data from sequencing 120 samples of 16S sequencing. I don't know yet how big the file is. I was wondering if it is possible.

Hello Laila,

I think the biggest limitation is your RAM / memory. Some of the initial steps like denoising can take more memory, so finding a machine with 16 GB (or more) could be helpful. Most of the downstream plugins for visualization and stat tests should work just fine with 8 GB.

You could also take a look at this post by Justine outline some great resources that could help you process data with the machine you have.


Thank you Colin! I'll check out the resources you shared.

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