Hi,
I recently got Illumina-based 16s rRNA sequencing data.
I am trying to import my data using Fastq manifest format.
and the demultiplexed data is looking unusual.
"SingleEndFastqManifestPhred33V2" this variant was used to the procedure.
I am yet to finish the trimming process.but, when I am visualising it, the quality score graph looks weird.
why is it,
can somebody please assist me,
the quality score graph is attached below

