When I used rescript to filter the low quality DNA sequence transformed by silva138, it suddenly reported an error, what might cause this?

qiime rescript cull-seqs --i-sequences silva-138.1-ssu-nr99-seqs.qza --o-clean-sequences silva-138.1-ssu-nr99-seqs-clean
Plugin error from rescript:

[Errno 2] No such file or directory: '/tmp/qiime2/wanglab/data/bd260170-a6cf-4fff-aa43-3e871c1b62b1/provenance'

Debug info has been saved to /tmp/qiime2-q2cli-err-ds99ade5.log

Hi @Xiang,

It appears that your system is unable able to access the temporary file paths for some reason. Sometimes the issue is due to the system limiting the available storage space in the temporary directory.

You can get around this by setting up a new tmp path to a place where you have more storage space. That is, you can run the following command in the terminal, prior to running your QIIME 2 commands, or simply add to your .bashrc file:

export TMPDIR='/home/username/tmp'

where username is your account user name.

Give this a try and let us know if it helps.

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