What was the model used for the PERMANOVA (e.g. free permutations of residuals - n=5 does not allow for many permutations at the lowest level)?

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Hi @YuZhang,

This is more a note of caution about your design and being careful with your beta diversity.

There isn't a good solution for this problem after samples have been collected and sequenced. The best time time to solve this is during the experimental design phase. The tests carry the sample size limitations they have and you're ultimately paying an FDR penalty if you're doing untargeted testing.

If you run into the isssue when you get there, here are a few threads on the subject

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