What methods can you recommend I use to assess the accuracy of the classifier I made?

[SoilRotifer (Mike Robeson)]

Hi @pkmnsandy,

@pkmnsandy

However, it requires me to use a known database already.

This is not true. Check out some of the tutorials listed on the github page. Some examples of interest are within the SILVA tutorial and this notebook.

@pkmnsandy

In my case, there is almost "not" known good classifier files for pmoA gene.

Several features in RESCRIPt are specifically for the purpose of evaluating your novel reference database. You can read more about it here.

-Cheers!