what is dada2 message mean

Hello

I am running 250 samples, and I get the following message from dada2.

  1. Learning Error Rates

“267211440 total bases in 1113381 reads from 5 samples will be used for learning the error rates”

What does this mean?

Does that mean poor sequencing quality?

Thank you.

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Hello @kimshinseung,
Getting this message when running dada2 is normal. Colin explains this really well in this post:


what this message is saying is that dada2 is basically training your error models with 267211440 total bases in 1113381 reads from 5 samples.
As long as you are getting an the correct output files from dada2 then you are golden :smile:

Hope this helps!
Chloe :turtle:

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