Vsearch dereplication plugin error

User Support
2

Hello @lucky_endophyte,

This seems to be a bug that hasn't been addressed yet, discussed here. I'll open an issue for this and we'll look into it. In the meantime I think you can achieve the same output with the following steps:

  • remove the --p-min-unique-size 10 parameter from the dereplicate-sequences call (it should then work)
  • filter the resulting feature table with feature-table filter-features using --p-min-frequency 10
  • filter the resulting representative sequences with feature-table filter-seqs using --i-table <your feature table>