V3-V4 silva classifier

@Wenxuan_Dong,

If you download those files, you can look at the provenance information by dragging and dropping them onto QIIME 2 View.

You will see that these files have had some basic filtering performed:

  • cull-seqs on the full length data
  • filter-seqs-length-by-taxon
  • dereplication

I will say, that if you are interested in making an amplicon-region specific classifier You probably do not need to run filter-seqs-length-by-taxon. Just dereplicate, extract the region, then cull / dereplicate those sequences. See this example post.

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