Hello everyone,
I just read this tutorial which indicates how to use GNEISS to evaluate the effect of continuous numerical variables in the microbiome, but apparently Gneiss has been deprecated in more recent versions of QIIME2. Is there an alternative in 2024.10 to run an analysis like gradient-clustering? (Which was qiime gneiss gradient-clustering in previous versions)
Thank you!
Hi @PabloCL,
Have you looked into q2-vizard at all? It might be what you are looking for:
We also have a tutorial for longitudinal analysis that might be helpful to you as well!
--Hannah
Hi, @jphagen, I actually didn't know about q2-vizard. but unfortunately it doesn't seem to be what I am looking for my dataset. I am particularly searching for a test that allows me to see how a numerical independent variable (in my case, soil temperature) influences changes in microbial communities. I particularly want to see which ASVs become more abundant at higher temperatures (and lower temperatures), and gradient-clustering seemed to be what I needed.
Thank you very much for the useful links though! I will make sure to keep them in mind for the future!
--Pablo