Dear all,
I’m interesting in analyzing the unique sequences found in fasta file that indicates sequences that are identical to the reference sequence. How could I extract the unique sequences?
Thank you!
Dear all,
I’m interesting in analyzing the unique sequences found in fasta file that indicates sequences that are identical to the reference sequence. How could I extract the unique sequences?
Thank you!
Hi @teleos,
Assuming that your fasta consists of unique sequences (ASVs or OTUs) output from one of the denoising/dereplication/otu picking methods in QIIME2, you can extract sequences that are identical to reference sequences by using exclude-seqs. Just set the --p-perc-identity
parameter to 1.0 to extract exact matches.
I hope that helps!
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