Hello all,
I am facing a similar problem when importing single end sequences in the moving pictures tutorial. I followed the following query and was successful to some extent. However, I am getting a different error now. I am attaching a pic of the code for more information. Can anyone provide any solutions? I am unable to import the data even if I created a desktop file named "Emp-Single-End-Sequences" and downloaded and extracted all the files related to the tutorial. I think I am missing something. qiime|690x240
I was able to find a partial solution for my problem here
This is one of those problems that's hard to spot because your file locations are almost perfect, but not quite 100% perfect. Hopefully we can get this fixed.
Based on your post, I think your files are set up like this:
folder you are in: emp-single-end-sequences
(file) 'Moving Pictures sample-metadata Qiime 2 2019.10 - sample-metadata.tsv'
(file) barcodes.fastq
(folder) emp-single-end-sequences
(file) sequences.fastq
The files are green and the folders are blue.
... Wait, it looks like you have two emp-single-end-sequences folders.
I have a strong suspicion that if you start over fresh (delete everything you have done so far) and follow the directions exactly, you will be fine. One thing I like to remember is that computers aren't clever or smart - they don't "fill in the blanks" when you make a mistake, they will just do whatever you told them to do, until it doesn't work anymore. I think that might be what happened here - one little mistake on your part when you first started has cascaded into a handful of confusing errors.
Please carefully review the documentation and follow each step precisely (countless people have preceded you on these steps, you can rest assured that it does work as advertised).
I think @thermokarst's advice is sound: the moving pictures tutorial should run like a cooking show. But, here's my theory:
In your folder, you have barcodes.fastq and sequences.fastq, but in your command, the error message is looking for sequences.fastq.gz. You've gunzipped your sequences somewhere along the way, and that's why this isn't working.
Thank you @jwdebelius@colinbrislawn@thermokarst it worked. Actually I unzipped the file. I removed both the unzipped file and the raw tsv file. And only kept the two sequence.fastq.gz and barcode.fastq.gz zipped file in the emp-single-end-sequences folder in Desktop. Once I ran the command it worked seamlessly.
