Trouble with q2-gneiss and the formula parameter

User Support
1

Hello!
I am new to qiime2 and this type of data analysis in general, so Im sure this will be a silly question.

I am working through gneiss and am currently stuck on generating the regression_summary.qzv. My sample metadata is really simple it has two categories, sampled and replicate. I was using the --p-formula “sampleid+replicate” but I get this error

Plugin error from gneiss:

Error evaluating factor: NameError: name ‘Replicate’ is not defined
sampleID+Replicate
^^^^^^^^^

I am sure I missed something or have made an error elsewhere, I am just not sure where.

I really appreciate your help here, Im stuck!

Thanks so much!
Scott

3

Can you post the header of your metadata file and the exact command that you ran? Chances are it is just a mispelling.

Also, is there any reason why you are testing for differences in sampleID? This is likely to lead to a overparameterized / nonsensical model.

5

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