Trouble with golay setting when demuxing reads

[KasturiChakraborty]

Hi,
I am having some problems with my demux.qza file. I tried using the following commands:

qiime demux emp-paired
–i-seqs emp-paired-end-sequences.qza
–m-barcodes-file sample-metadata.tsv
–m-barcodes-column BarcodeSequence
–o-per-sample-sequences demux.qza
–o-error-correction-details demux details.qza
–p-rev-comp-mapping-barcodes

The same data set gives me a 120MB demux.qza file on qiime2. 2019.1 where as it gives me just a 3.6MB file on qimme2. 2019.4.
Not sure what do I need to change.

[thermokarst]

Hi @KasturiChakraborty! Do you have golay barcodes? If not, you will need to disable the golay error correction with --p-no-golay-error-correction parameter. Automatic golay correction was added in 2019.4. Let us know how it goes!