Trouble installing Qiime2 2020.8 environment -- issue with Seaborn

Hello everyone,

I am trying to work with EzMap for the first time and I am having some difficulties installing Qiime2.

In trying to install mincodona and qiime2 on my own, I was able to install miniconda3 with help from the following forum: Installing Miniconda but I am having trouble installing the qiime2 environment (Installing QIIME 2) This is the error I am receiving:

Could not solve for environment specs
The following packages are incompatible
├─ q2-sample-classifier 2020.8.0** is installable and it requires
│ └─ seaborn >=0.8 , which can be installed;
└─ seaborn-base 0.10.1** is not installable because it requires
└─ seaborn 0.10.1 *_1, which conflicts with any installable versions previously reported.

I have also tried to install qiime2 from the EzMap Java interface but I am told it cannot find the environment, which makes sense. However, I did try to install qiime2 this way before and it seemed to work (I had different issues down the line so I scrapped everything and started over.) This is the error I am getting here:

bash ~/Desktop/EzMAP/scripts/installqiime.sh

(base) minnaschmidt@MacBookPro ~ % source activate qiime2-2020.2 &&

cmdand>

cmdand> bash ~/Desktop/EzMAP/scripts/installqiime.sh

EnvironmentNameNotFound: Could not find conda environment: qiime2-2020.2

You can list all discoverable environments with conda info --envs.

Thank you so much and all the very best!

~Minna.

Hello @MSchmidt,

Can you post the contents of the installqiime.sh file? If that created a conda environment, it apparently isn't called "qiime2-2020.2", as the EnvironmentNameNotFound error indicates. Is there a reason you're trying to create an environment that's 4 years old? If not, your best bet is to install the most recent version--instructions for doing so are here.

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Hi @colinvwood,

Thanks so much for your response! I am trying to follow the instructions from the EzMap publication in order to first work with the data they give as an example in Github (I am just starting out as a beginner and don't have coding experience.) This is the link to the publication: EzMAP: Easy Microbiome Analysis Platform | BMC Bioinformatics | Full Text

I have tried to provide the contents of the installqiime.sh file but I am getting an error message that new users can only provide 2 links

Here is a screenshot of what I am seeing when I open the file. Is this the correct information?

Thank you so much and all the very best!

Hello @MSchmidt,

As a first step I would just try to install the latest version of qiime2 from the link I provided and then run the two wget commands that download classifiers and the two mv commands separately--I'm assuming you need these for the tutorial you're following. If it turns out that you absolutely have to have an older version of qiime2 we can circle back and try to figure that out.

Hello @colinvwood ,

Thank you so much! I did previosuly try to download the latest qiime2 from the link when I was trying to troubleshoot myself but I ran into issues at the time as well. I have currently installed all of the environments mentioned in the article (aplicon, metagenome, and tiny.) If possible, could you help direct where where instructions for running the wget and mv files could be, as well as how to run them separately.

All the very best!

Hello @MSchmidt,

Those commands are lines 17-28 in the file that you screenshotted. Just run those in your terminal.

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