Hi all. I'm new to Qiime and hope to get some help here. I did the demultiplexing and got the result as shown in the figure. How can I trim the sequence only for reverse one as shown in the red cicle (this is a paired-end sequencing) as I don't know the sequence base pair at all? Sorry if I use the wrong term and Thank you!
Welcome to the forum, @alexp!
What is your objective in trimming those positions? If you're just trying to get rid of low-quality biological data, and are planning to denoise with DADA2, the denoise-paired command will let you trim/truncate the 5' or 3' end of your reads as you like before denoising. Details in the docs.
Best,
Chris 
Thank you @ChrisKeefe !
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