I am trying to run PacBio CCS amplicon data in qiime2-2023.7 but present data is in the following format "A10.hifi_reads.fastq-gz; A5.hifi_reads.fastq-gz", hence, I could not import it into qiime. I understand i need the data to be in SingleEndFastqManifestPhred33V2 formt. Is there a way to convert this data to Qiime 2 manifest format importable into qiime2.
error found below-
(qiime2-2023.7) sam@Samss-MacBook-Air mohammad % qiime tools import
--type 'SampleData[SequencesWithQuality]'
--input-path /Users/sam/OfoeFull16S\ copy
--output-path reads_qza/raw_reads.qza
--input-format SingleEndFastqManifestPhred33V2
There was a problem importing /Users/sam/OfoeFull16S copy:
/Users/sam/OfoeFull16S copy is not a file.