Yes i followed the RESCRIPt plugin install instructions, option 2
with no problem and I am following until make our classifier for use on full-length SSU sequences. now as follows
time qiime feature-classifier fit-classifier-naive-bayes --i-reference-reads silva-138-ssu-nr99-seqs-derep-uniq
.qza --i-reference-taxonomy silva-138-ssu-nr99-tax-derep-uniq.qza --o-classifier silva-138-ssu-nr99-classifier.qza Killed
Why I receive killed after several trials with this step please? I have 15.8 GB memory and it is always killed after reaching 13 GB of the memory!! What should i do so it is not killed every time please
For later steps, i received from sequencing center an email with primers used, message includes **illumina primer and primer, which one is the one intended to be used? I deal with v3/v4 16S
Often users require upwards of 32-64 GB of RAM to train the full-length sequence SILVA classifier. If you are going to use the full-length classifier, you can simply use the one we provide here. You might be able to construct the V3V4 region specific classifier with 16 GB RAM as it 'll be much smaller.
If you perform an internet search for "V3V4 primer sequence" you'll find that those listed in the Primer column are the primers you'd use as input into cutadapt.