In the code below:
qiime feature-classifier extract-reads
If we trimmed the forward and reverse sequences differently at the dada2 step, what --p-trunc-len should we use here? So for me, the forward read was not cut and stayed at 250nts, while the reverse was lower quality and so was cut at 244.
Also, how do we choose values for the --p-min-length and --p-max-length?