Hi @Muhammad! You’re using QIIME 2 version 2017.2 – you’ll need to upgrade to version 2017.4 and rerun the qiime demux summarize command to get access to the new interactive quality score plots.
Thank for the suggestion. I upgrade to the 2017.4 version. This time i got the file quality-plot.html. But there was no plot. I open the html file and show the below information (file:///home/qiime2/.cache/.fr-m7rGxY/20a40871-e2b5-48a7-9642-371b9fd19c8c/data/quality-plot.html).
Click and drag on plot to zoom in. Double click to zoom back out to full size.
Hover over a box to see the parametric seven-number summary of the quality scores at the corresponding position.
Forward Reads
Reverse Reads
These plots were generated using a random sampling of 10,000 out of 1,306,300 sequences without replacement. Outlier quality scores are not shown in box plots for clarity.
Hi @Muhammad, can you please provide the commands that you used to generate and view the plot? Can you send a screenshot of what you are seeing?
You should be able to view your visualizations at https://view.qiime2.org, or by running qiime tools view <FILENAME>.qzv on the command line (assuming you aren’t running on a headless server).
Judging from your screenshot, it looks like you are double-clicking demux.qzv, unzipping it (the unzip tool is highlighted in your sidebar), and then you are opening the html files directly. This is not the recommended way of viewing visualizations - please take a look at the demux section of the "Moving Pictures" tutorial for more info on viewing visualizations:
So, as I mentioned in my last message, you should be able to view demux.qzv by opening the file at https://view.qiime2.org, or by running qiime tools view demux.qzv.