The Silva classifier I trained was used for fungal, but the species classification results were all bacteria

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Hello, :grinning:

I'm sorry to keep bothering you. I selected 21 sequences from the sample and uploaded them to blast for comparison.

First, I used the nucleoside collection database comparison. seqdump.txt (31 Bytes)

I checked the distance tree of results file. The results are shown in the figure below, and the one marked in red is eukaryote.

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undefined1737×812 242 KB

And then, I compared the same batch of data with 18S ribosomal RNA sequences (SSU) from functional type and reference material database.fungal-seqdump.txt (179.5 KB)

I checked the distance tree of results file. The results are shown in the figure below, and the one marked in red is fungal.

18S
18S1267×837 252 KB

This is my first time using blast. I am puzzled whether this result can show that the primers selected for this batch of 18S fungi data are not suitable.

I really hope you can give me some help. Thank you. :pray: