I was trying to find out the features differ in abundance across different groups with ANCOM following “Moving picture tutorial”.
ANCOM assumes that few (less than about 25%) of the features are changing between groups. If you expect that more features are changing between your groups, you should not use ANCOM as it will be more error-prone (an increase in both Type I and Type II errors is possible).
I would like to know the details of this one sentence because I couldn’t
understand what it meant.
If someone would be so kind , could you please explain it clearly ?