Is there any difference between [PairedEndFastqManifestPhred33] and [PairedEndFastqManifestPhred33V2]?
It seems there is no difference, just used for different qiime2 version
The V2 actually corresponds with the version of the manifest, rather than the Phred score.
Early on in QIIME 2 days the manifest format was slightly different than what it is now, for example, the current (V2) format is a tab separate text file structured like so:
sample-id forward-absolute-filepath reverse-absolute-filepath sample-1 $PWD/some/filepath/sample0_R1.fastq.gz $PWD/some/filepath/sample1_R2.fastq.gz sample-2 $PWD/some/filepath/sample2_R1.fastq.gz $PWD/some/filepath/sample2_R2.fastq.gz sample-3 $PWD/some/filepath/sample3_R1.fastq.gz $PWD/some/filepath/sample3_R2.fastq.gz sample-4 $PWD/some/filepath/sample4_R1.fastq.gz $PWD/some/filepath/sample4_R2.fastq.gz
While the previous manifest versions was a comma separated file structured like so:
sample-id,absolute-filepath,direction sample-1,$PWD/some/filepath/sample1_R1.fastq,forward sample-1,$PWD/some/filepath/sample1_R2.fastq,reverse
The newest version is the recommended version to use but the old format I believe was kept as a legacy format.
Hope this clarifies things.
I got it! Thanks a lot!
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