Taxonomy file has lines not in sequences file

[John]

Hello,

I passed a large database containing the entire ITS region through ITSx to isolate the ITS2 region. The process generated an ITS2 file that is missing some of the original fasta lines because it did not find the region. I have a taxonomy file that corresponds to the original database, which of course contains all the entries before filtering. Is it OK that the taxonomy file contains entries that are not in the corresponding sequences file? If not, is there a tool to do this or will I have to write a script to match and remove?

John

[Nicholas_Bokulich]

Hi @John ,

@John

Is it OK that the taxonomy file contains entries that are not in the corresponding sequences file?

Yes this is not a problem (and is quite typical, actually, after filtering out sequences for one reason or another). The extra lines in the taxonomy will just be ignored by most actions.

If you ever run into a situation where the taxonomy and sequences must match exactly (there are a few rare actions that require this), you can use RESCRIPt to filter your taxonomy as shown in this tutorial (see the filter-taxa action):

Using RESCRIPt to compile sequence databases and taxonomy classifiers from NCBI Genbank

This tutorial will describe how to create custom reference databases from NCBI Genbank using RESCRIPt. Read more about RESCRIPt here: Processing, filtering, and evaluating the SILVA database (and other reference sequence data) with RESCRIPt - #7 If using NCBI Genbank data, please be aware of the NCBI disclaimer and copyright notice. Citation: If you use RESCRIPt or any RESCRIPt-processed data in your research, please cite the following pre-print: Michael S Robeson II, Devon R O'Rourke, Benj…