Hello, All, I want to create customized database for analysing bacterial 16srRNA sequence data, I have downloaded NCBI REF-seq 21,789 16srRNA sequence. could you please guide me how to create taxonomy txt file for this 21,789 sequence Is there any method or coding to create taxonomy file for 21,…

Hi @Asha1 , [image] Asha1: Is there any method or coding to create taxonomy file for 21,789 sequence rather than manually? Yes, there is a new QIIME 2 plugin that will do this for you. We have a tutorial for it on this forum: [image] Using RESCRIPt to compile sequence …

Thank so much for your reply sir. I owe you a lot sir As per your suggestion, I have installed rescript and ran the following command line "qiime rescript get-ncbi-data --p-query singleline2.fasta --o-sequences ncbi-refseqs-unfiltered.qza --o-taxonomy ncbi-refseqs-taxonomy-unfiltered.qza" In -p q…

Hi @Asha1 , The command you are using is not really following the tutorial I sent you before... that tutorial gives the exact instructions you need to build a classifier on NCBI-Refseqs 16S. The error you are getting is precisely because you are inputting an improperly formatted sequence file as me…

Thank so much for your reply sir. I have finished creating the customised database. data.tsv (476 Bytes) [visualization] Please tell me can I proceed my further data analysis with this classifier?. Classifier performance is good or not sir?

[image] Asha1: I have finished creating the customised database. Quick, wasn't it :smile: that classifier can now be used to classify other sequences. [image] Asha1: Please tell me can I proceed my further data analysis with this classifier?. Classifier performance is good or not sir? …

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