I want to create customized database for analysing bacterial 16srRNA sequence data, I have downloaded NCBI REF-seq 21,789 16srRNA sequence. could you please guide me how to create taxonomy txt file for this 21,789 sequence
Is there any method or coding to create taxonomy file for 21,789 sequence rather than manually?
is it mandatory to give all level of taxonomy ( Kingdom to species) or geneus and species name is enough for running in qiime?
we have sequenced only v3-v4 region for our bacterial sample. Now, I have download whole 16srRNA sequence, is it mandatory to pick only v3-v4 target region alone from the sequence or could I use entire 16srRNA sequence for analysis in qiime? Using the entire 16srRNA sequence affect the analysis or not?