Thank so much for your reply sir. I owe you a lot sir
As per your suggestion, I have installed rescript and ran the following command line “qiime rescript get-ncbi-data --p-query singleline2.fasta --o-sequences ncbi-refseqs-unfiltered.qza --o-taxonomy ncbi-refseqs-taxonomy-unfiltered.qza”
In -p query options I gave my ncbi refseq fasta file after converting all the sequence in to single line.
I am getting the following error
Plugin error from rescript:
Taxonomy format requires at least one row of data.
Debug info has been saved to /tmp/qiime2-q2cli-err-x9a_zcsl.log
I got one doubt do I have to create ncbi-refseqs-unfiltered.qza and refseqs-taxonomy-unfiltered.qza separately or this plugin will generate?
If want to create mean, I have fasta sequence alone. How to download the taxonomy file for the 33175[BioProject] OR 33317[BioProject]. I didnt get taxonomy file for this id in NCBI taxonomy database.
kindly help me