taxonomic classification with two marker genes in one fasta file

Hi @Alfred.Burian,

I don't have a good database recommendation, but you might look at using cutadapt to slip the sequences, using the primers, using the --p-untrimmed parameter. (Note that this only works if the sequencing center hasn't trimmed your primer sequence; but it's worth trying).

One example of that discussion is here:

You might also searching for cutadapt on the forum for more discussion.

Best,
Justine