Taxanomyanalysis error


I have queries regarding taxanomy analysis. when I performed taxanomy analysis with SILVA_132_QIIME_release. I am getting the following errror continously

If I perform taxanomy analysis with customized database also, I am getting that white space error

Herewith I have attached taxonomy analysis result file link also for your perusal

Could anyone give suggestion how to rectify this error?

Hi @Asha1,

The error message is pretty clear: It looks like you have a white space in your taxonomy file. I would search for white spaces in your taxonomy files and strip them out either manually by finding and replacing them or using a β€œstrip” functionality in R or Python.


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After removing white space also, I am getting error again

I have attached files link for your perusal

kindly help me how to solve this error?

Hi @Asha1,

Could you please share the contents fo the debug file? Post it as text, rather than attaching it.


Thank so much for ur prompt reply. I have given debug file error and files for your perusal.
(qiime2-2019.1) [email protected]:~/miniconda2/envs/qiime2-2019.1/v3-v4_region_result/99/manoj/cd_result$ qiime feature-classifier fit-classifier-naive-bayes --i-reference-reads single.qza --i-reference-taxonom
y cd.qza --o-classifier classifier.qza --verbose
/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_feature_classifier/ UserWarning: The TaxonomicClassifier artifact that results from this method was trained using scikit-learn version 0.
20.2. It cannot be used with other versions of scikit-learn. (While the classifier may complete successfully, the results will be unreliable.)
warnings.warn(warning, UserWarning)
Traceback (most recent call last):
File β€œ/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/q2cli/”, line 274, in call
results = action(**arguments)
File β€œ</home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/>”, line 2, in fit_classifier_naive_bayes
File β€œ/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/”, line 231, in bound_callable
output_types, provenance)
File β€œ/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/qiime2/sdk/”, line 365, in callable_executor
output_views = self._callable(**view_args)
File β€œ/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_feature_classifier/”, line 316, in generic_fitter
File β€œ/home/ruthra/miniconda2/envs/qiime2-2019.1/lib/python3.6/site-packages/q2_feature_classifier/”, line 31, in fit_pipeline
y, X = list(zip(*data))
ValueError: not enough values to unpack (expected 2, got 0)

Plugin error from feature-classifier:

not enough values to unpack (expected 2, got 0)

cd.txt (3.5 KB)

file link

Thanking you once again for your suggestions and help. Looking forward to your reply

Your taxonomy database is not formatted properly. See this post (and others in the forum archive with the search functionality) for help troubleshooting:

Good luck!