Subject:[QIIME2.2023.5] Need help with Qiime2 installation: ResolvePackageNotFound error

Subject: Need help with Qiime2 installation: ResolvePackageNotFound error

Dear Qiime2 Community,

I hope this message finds you well. I am currently facing an issue during the installation of Qiime2 and would greatly appreciate your assistance in resolving it.

During the installation process, after following the Qiime2 instructions, I encountered the following error:

diff

Collecting package metadata (repodata.json): done
Solving environment: failed
- scikit-bio=0.5.7                                                                                    - provenance-lib=2023.5.1                                                                             - libgpg-error=1.46                                                                                   - r-base=4.2.3                                                                                        - gcc_impl_linux-64=12.2.0                                                                            - q2-phylogeny=2023.5.0                                                                               - r-xml2=1.3.4                                                                                        - q2-taxa=2023.5.0                                                                                    - bioconductor-sparsematrixstats=1.10.0                                                               - bioconductor-xvector=0.38.0                                                                         - bioconductor-shortread=1.56.0                                                                       - q2-feature-classifier=2023.5.0                                                                      - q2-longitudinal=2023.5.0                                                                            - bioconductor-decipher=2.26.0                                                                        - htslib=1.17                                                                                         - libsanitizer=12.2.0                                                                                 - fasttree=2.1.11                                                                                     - q2-feature-table=2023.5.0                                                                           - libedit=3.1.20191231                                                                                - sed=4.8                                                                                             - libxkbcommon=1.5.0                                                                                  - libuuid=2.38.1                                                                                      - q2-dada2=2023.5.0                                                                                   - q2-cutadapt=2023.5.1                                                                                - libstdcxx-ng=12.2.0                                                                                 - libsystemd0=253                                                                                     - bioconductor-dada2=1.26.0                                                                           - perl-compress-raw-zlib=2.202                                                                        - xcb-util=0.4.0                                                                                      - bioconductor-iranges=2.32.0                                                                         - q2-vsearch=2023.5.0                                                                                 - alsa-lib=1.2.8                                                                                      - nspr=4.35                                                                                           - gfortran_impl_linux-64=12.2.0                                                                       - r-mass=7.3_60                                                                                       - sepp=4.3.10                                                                                         - bioconductor-genomicalignments=1.34.0                                                               - libgcc-ng=12.2.0                                                                                    - libunistring=0.9.10                                                                                 - xcb-util-renderutil=0.3.9                                                                           - nss=3.89                                                                                            - hmmer=3.1b2                                                                                         - bioconductor-dirichletmultinomial=1.40.0                                                            - emperor=1.0.3                                                                                       - entrez-direct=16.2                                                                                  - bioconductor-s4vectors=0.36.0                                                                       - bioconductor-rhdf5lib=1.20.0                                                                        - q2-fragment-insertion=2023.5.0                                                                      - binutils_impl_linux-64=2.40                                                                         - q2-mystery-stew=2023.5.0                                                                            - attr=2.5.1                                                                                          - libev=4.33                                                                                          - bioconductor-rsamtools=2.14.0                                                                       - libidn2=2.3.4                                                                                       - bioconductor-beachmat=2.14.0                                                                        - q2galaxy=2023.5.0                                                                                   - tktable=2.10                                                                                        - bioconductor-zlibbioc=1.44.0                                                                        - unifrac-binaries=1.1.1                                                                              - libgfortran5=12.2.0                                                                                 - bioconductor-biocsingular=1.14.0                                                                    - unifrac=1.1.1                                                                                       - q2-types=2023.5.0                                                                                   - _openmp_mutex=4.5                                                                                   - libgcrypt=1.10.1                                                                                    - blast=2.14.0                                                                                        - gmp=6.2.1                                                                                           - bioconductor-scuttle=1.8.0                                                                          - bioconductor-biocparallel=1.32.5                                                                    - xcb-util-image=0.4.0                                                                                - q2-composition=2023.5.0                                                                             - q2-metadata=2023.5.0                                                                                - q2-alignment=2023.5.0                                                                               - bioconductor-biostrings=2.66.0                                                                      - perl-json-xs=2.34                                                                                   - perl-io-compress=2.201                                                                              - perl-list-moreutils-xs=0.430                                                                        - q2-quality-control=2023.5.0                                                                         - libstdcxx-devel_linux-64=12.2.0                                                                     - q2templates=2023.5.0                                                                                - libnghttp2=1.52.0                                                                                   - ncurses=6.3                                                                                         - bioconductor-rhdf5filters=1.10.0                                                                    - keyutils=1.6.1                                                                                      - bioconductor-multtest=2.54.0                                                                        - dbus=1.13.6                                                                                         - libcap=2.67                                                                                         - perl-pathtools=3.75                                                                                 - libgcc-devel_linux-64=12.2.0                                                                        - iow=1.0.5                                                                                           - q2-deblur=2023.5.0                                                                                  - samtools=1.17                                                                                       - xcb-util-wm=0.4.1                                                                                   - vsearch=2.22.1                                                                                      - libgomp=12.2.0                                                                                      - perl-compress-raw-bzip2=2.201                                                                       - q2-gneiss=2023.5.0                                                                                  - bowtie2=2.5.1                                                                                       - bioconductor-biobase=2.58.0                                                                         - qiime2=2023.5.1                                                                                     - sortmerna=2.0                                                                                       - bioconductor-delayedarray=0.24.0                                                                    - dnaio=0.10.0                                                                                        - xcb-util-keysyms=0.4.0                                                                              - perl-storable=3.15                                                                                  - readline=8.2                                                                                        - q2-demux=2023.5.0                                                                                   - libnsl=2.0.0                                                                                        - raxml=8.2.12                                                                                        - q2-diversity=2023.5.1                                                                               - q2-emperor=2023.5.0                                                                                 - ld_impl_linux-64=2.40                                                                               - mafft=7.520                                                                                         - bioconductor-genomicranges=1.50.0                                                                   - bioconductor-rhtslib=2.0.0                                                                          - xkeyboard-config=2.38                                                                               - libcups=2.3.3                                                                                       - bioconductor-biocneighbors=1.16.0                                                                   - pulseaudio-client=16.1                                                                              - pbzip2=1.1.13                                                                                       - q2-sample-classifier=2023.5.0                                                                       - wget=1.20.3                                                                                         - perl-scalar-list-utils=1.63                                                                         - bioconductor-rhdf5=2.42.0                                                                           - libgfortran-ng=12.2.0                                                                               - cutadapt=4.4                                                                                        - q2cli=2023.5.1                                                                                      - gxx_impl_linux-64=12.2.0                                                                            - q2-quality-filter=2023.5.0                                                                          - q2-diversity-lib=2023.5.0                                                                           - iqtree=2.2.2.3      
...

My Conda version is v23.5.0, and I followed the provided instructions:

  1. Downloaded the Qiime2 distribution file using the wget command:
wget https://data.qiime2.org/distro/core/qiime2-2023.5-py38-linux-conda.yml
  1. Created a new Conda environment using the following command:
conda env create -n qiime2-2023.5 --file qiime2-2023.5-py38-linux-conda.ym
  1. The only thing I did differently was installing wget through this command:
conda install -c rmg wget

as opposed to using "conda install wget".

However, the ResolvePackageNotFound error persists.

I would be grateful if you could provide guidance on resolving this issue. If there are alternative methods or troubleshooting steps I can try, please let me know. Any assistance you can offer to help me successfully install Qiime2 would be highly appreciated.

Thank you very much for your time and support.

1 Like

Hello @qiime24thee, are you installing QIIME 2 on a native Linux install or on WSL? Additionally, have you tried installing again today? Errors like that are frequently transient. Assuming it is not transient, can you run conda clean --all from your base conda environment and say yes to everything then attempt the install again? That command won't remove anything from any of your environments, it will only remove some stuff conda has cached.

2 Likes

Fixed. For anyone who might lurk here in the future: Repeat the same installation steps until it works. It took a few days, but eventually it worked. Cool.

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