strange alpha rarefaction curve

Hello @tlin , and welcome to the :qiime2: Forum.

It is quite likely that your rarefaction curves are collapsed by metadata. Observed features seem to decrease probably because as sequencing depth grows, you are averaging over less samples (those with less depth than the sequencing depth are discarded). Try to generate by sample curves not aggregating by metadata.

Something similar was addressed in this post, if you want to take a look.

Best,

Sergio

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