Hello all,
I've produced this very strange rarefaction curve. Does anybody have an idea of what might have gone wrong? All but the yellow curve looks fine so I'm not sure what has happened.
Thanks in advance!
Best
Tommie
Hello @tlin , and welcome to the 
Forum.
It is quite likely that your rarefaction curves are collapsed by metadata. Observed features seem to decrease probably because as sequencing depth grows, you are averaging over less samples (those with less depth than the sequencing depth are discarded). Try to generate by sample curves not aggregating by metadata.
Something similar was addressed in this post, if you want to take a look.
Best,
Sergio
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