Statistical analysis

[Nicholas_Bokulich]

Hi @tengfey,

@tengfey

For ANCOM, are there commands to visualize the taxonomic distribution that are significant in a given group, such as the boxplot/histogram

Not currently. That specific visualization would need to be done outside of QIIME2.

@tengfey

Moreover, what is the meaning of W values and is that insert the text relevant to the explanation of “ANCOM Volcano Plot”?

See this thread

@tengfey

Are there commands for the statistical methods to compare the metrics and relative abundance among two and more groups, such as ANOVA and t-tests) or non-parametric (Kruskal-Wallis, Wilcoxon) or LEfSe?

See this thread. For differential abundance testing, ANCOM is what you are asking for. Pairwise testing with ANCOM is one approach to compare two groups (similar to running pairwise t-tests or WIlcoxon tests).

For other metrics, e.g., alpha and beta diversity, yes. Appropriate multi-group and pairwise tests (e.g., Kruskal Wallis) are used by default — see the moving pictures tutorial for more details.

I hope that helps!