sir i have 2 fastq sequences for one sample R1 and R2. and i don't have other information such as barcode and all. my question is how i can upload my this sequence in qiime 2 for analysis? i tried with Parkinson mouse tutorial but when i upload my data it showing error . i am attached screenshot of…

Hi @SAMRENDRA01 ! Two issues: First, you appear to be providing a TSV manifest to, but using the old V1 manifest format. You should change the --input-format to read PairedEndFastqManifestPhred33V2 (note the V2 at the end). Second, you have an extra space in the first column's header name, right a…

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sir i m facing problem to importing my demultiplexed data in qiime 2