sir i have 2 fastq sequences for one sample R1 and R2. and i don't have other information such as barcode and all. my question is how i can upload my this sequence in qiime 2 for analysis?
i tried with Parkinson mouse tutorial but when i upload my data it showing error . i am attached screenshot of the erors
First, you appear to be providing a TSV manifest to, but using the old V1 manifest format. You should change the --input-format to read PairedEndFastqManifestPhred33V2 (note the V2 at the end).
Second, you have an extra space in the first column's header name, right after the sample-id - delete that and you should be set.