Similarity when sequence of ASV is aligned to PICRUSt2 reference sequence

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1

Hello,

I am trying to analysis the picrust2 result using qiime2 output.

I wonder how much similarity is the standard when representative sequence of ASV is aligned to PICRUSt2 reference sequence. If --min-align is an option corresponding to similarity, I wonder what the default is.

Thanks a lot

2

Hi @songej486,
Welcome to the :qiime2: forum!
The default value is 0.8. I found this by running qiime picrust2 full-pipeline --help.

Screenshot 2023-05-15 at 10.25.17 AM
Screenshot 2023-05-15 at 10.25.17 AM1186×208 69.5 KB

Hope that helps!
:turtle:

3

Hi @cherman2
Thank you very much for your answer.
It was of great help to me.