silva reference files not available on qiime2 website

[Siyu_Song]

Hi all,

I'm trying to download the two Silva reference sequence and taxonomy files that were processed using [RESCRIPt] on the website Data resources — QIIME 2 2024.5.0 documentation. However, it seems like the two files:

• Silva 138 SSURef NR99 515F/806R region sequences (MD5: a914837bc3f8964b156a9653e2420d22)
• Silva 138 SSURef NR99 515F/806R region taxonomy (MD5: e2c40ae4c60cbf75e24312bb24652f2c)

are not actually accessible. It shows ERROR 404: NOT FOUND. when I try to use wget command to access the files. Could anyone try to download these two files on the website and see if the files could actually be downloaded?

Thank you!

[Nicholas_Bokulich]

Hi @Siyu_Song ,
Another forum member discovered this recently — please see the discussion on this topic:

Q2-compatible Silva reference sequences 404 not found

Oh gosh, you're right! Sorry about that! It appears that the files are not included in the latest release... not sure why. You can download them from the prior release here Just be sure to click the "Show me content on this page". The files are identical, but you'll have to train the classifier for 2024.5 based on these input files. I'd recommend simply using RESCRIPt as outlined here, if you'd like to minimize any of the curation that was performed. Depending on what you ar…

Thanks!