Good morning, I’m a new user and I am having problems with taxonomical assignment. I’m running Qiime2 installed with VirtualBox. The sequences that I have imported were already demultiplexed and I’ve used dada2 for the denoise and joining.
My question is how much RAM does the taxonomical assignment require. What I have tried to use is the Silva classifier found on the QIIME2 web site.
Silva 138 99% OTUs full-length sequences (MD5:
I have just 12 joined and demultiplexed samples and I have provided 10G to the virtual box, but when I start the taxonomy assignment process it doesn’t work, and I get an error saying that it needs more memory.
I was wondering if there is a minimum amount of RAM for this classifier to work properly, because in the future I will need to run the process with more samples and I can’t even get it with 12.
Any help would be welcome.
Thank you so much!