I want to create a database using SILVA_132, using only 16S sequences.
I have read the RESCRIPt tutorial on creating the database starting from silva, but I cannot figure out how to create it from silva 132 compared to silva 138 shown in the example. If I download the Silva_132_relase.zip folder from the link Archive, I cannot understand how to import the content into qiime2 as in the first part of the tutorial ...
tax_slv_ssu_138.txt.gz taxmap_slv_ssu_ref_nr_138.txt.gz tax_slv_ssu_138.tre.gz the sequence file: SILVA_138_SSURef_Nr99_tax_silva_trunc.fasta.gz
what should i use ???
Thanks for your help